Open FLIM software and standards

Lifetime analysis is only as trustworthy as the software and calibration behind it. I contribute to open FLIM analysis tools and to community efforts on quality control.

Software

• flimj-ops: ImageJ Ops plugins for fluorescence lifetime image analysis.
• Decay ↔ phasor explorer: an in-browser teaching tool for phasor analysis.

Methods

• Nonparametric empirical Bayesian framework for fluorescence-lifetime imaging microscopy. Biomed. Opt. Express 10, 2019.
• Fluorescence lifetime: techniques, analysis, and applications in the life sciences. Book chapter in Imaging from Cells to Animals In Vivo, 2020.

Community

• ERB member, QUAREP-LiMi, through Working Group 15 on fluorescence lifetime imaging (since October 2026).